Participating Faculty

Chengtao Her

Chengtao Her

Department:School of Molecular Biosciences, WSU
Credentials:1996 - Ph.D., Mayo Clinic/Mayo Medical School
Office:Biotech Lsf 343
Mailing Address:Washington State University
PO Box 647520
Pullman, WA 99164-7520

Research Interests

Genomics of Reproduction

Research Summary

My laboratory is interested in the application of the state-of-the-art genomics and proteomics approaches to address fundamental questions related to mammalian DNA mismatch repair pathways. In particular, we are interested in the understanding of the molecular mechanisms underlying mismatch repair genes in human cancer, as well as to study the roles of mismatch repair proteins in homologous recombination and other biological pathways facilitated by the identification and characterization of protein-protein and protein-DNA interactions. All current on-going projects in the laboratory utilize a combination of molecular biology/genomics, protein engineering/proteomics, and gene targeting approaches.

A separate research interest of the laboratory is to develop a green fluorescent protein (GFP)-based protein-protein interaction assay system. Proteins are the ultimate players involved in the vast majority of biological processes and in most cases their functions depend on specific protein-protein interactions. Obviously, one of the central focuses of the emerging field of proteomics is to develop multitasking assay systems for analyzing proteome-wide protein interactions.

Research Publications

Chu, Y. L., et al. (2013). "DNA damage induced MutS homologue hMSH4 acetylation." Int J Mol Sci 14(10): 20966-20982.

Chu, Y. L., et al. (2013). "MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair." Mol Cancer 12: 51.

Clark, N., et al. (2013). "MutS Homologues hMSH4 and hMSH5: Genetic Variations, Functions, and Implications in Human Diseases." Curr Genomics 14(2): 81-90.

Wu, X., et al. (2013). "MutS homologue hMSH5: recombinational DSB repair and non-synonymous polymorphic variants." PLoS One 8(9): e73284.

Xu, Y. and C. Her (2013). "VBP1 facilitates proteasome and autophagy-mediated degradation of MutS homologue hMSH4." FASEB J 27(12): 4799-4810.

Tompkins, J. D., et al. (2012). "MutS homologue hMSH5: role in cisplatin-induced DNA damage response." Mol Cancer 11: 10.

Xu, K., et al. (2012). "Assessment of anti-recombination and double-strand break-induced gene conversion in human cells by a chromosomal reporter." J Biol Chem 287(35): 29543-29553.

Wu, X., et al. (2011). "Causal link between microsatellite instability and hMRE11 dysfunction in human cancers." Mol Cancer Res 9(11): 1443-1448.

Tompkins, J.D., X. Wu, Y.L. Chu, and C. Her, "Evidence for a direct involvement of hMSH5 in promoting ionizing radiation induced apoptosis." Exp Cell Res, 2009. 315(14): p. 2420-32.

Yang, F., K. Gell, G.W. van der Heijden, S. Eckardt, N.A. Leu, D.C. Page, R. Benavente, C. Her, C. Hoog, K.J. McLaughlin, and P.J. Wang, "Meiotic failure in male mice lacking an X-linked factor." Genes Dev, 2008. 22(5): p. 682-91.

Zhao, N., F. Zhu, F. Yuan, A.K. Haick, S. Fukushige, L. Gu, and C. Her, "The interplay between hMLH1 and hMRE11: role in MMR and the effect of hMLH1 mutations." Biochem Biophys Res, 2008. 370(2): p. 338

Her, C., N. Zhao, X. Wu, and J.D. Tompkins, "MutS homologues hMSH4 and hMSH5: diverse functional implications in humans." Front Biosci, 2007. 12: p. 905-11.

Lee, T.H., W. Yi, M.D. Griswold, F. Zhu, and C. Her, "Formation of hMSH4-hMSH5 heterocomplex is a prerequisite for subsequent GPS2 recruitment." DNA Repair (Amst), 2006. 5(1): p. 32-42.

Yi, W., T.H. Lee, J.D. Tompkins, F. Zhu, X. Wu, and C. Her, "Physical and functional interaction between hMSH5 and c-Abl." Cancer Res, 2006. 66(1): p. 151-8.

Vo, A.T., F. Zhu, X. Wu, F. Yuan, Y. Gao, L. Gu, G.M. Li, T.H. Lee, and C. Her, "hMRE11 deficiency leads to microsatellite instability and defective DNA mismatch repair." EMBO Rep, 2005. 6(5): p. 438-44.

Yi, W., X. Wu, T.H. Lee, N.A. Doggett, and C. Her, "Two variants of MutS homolog hMSH5: prevalence in humans and effects on protein interaction." Biochem Biophys Res Commun, 2005. 332(2): p. 524-32.

Washington State University