Participating Faculty
Ed Schmidt
Name:Ed Schmidt
Department:Department of Microbiology and Immunology, MSU
Credentials:1990 - Ph.D., Oregon State University, Biochemistry & Biophysics
Phone:406-944-6375
Fax:406-944-4303
Mailing Address:Dept. of Microbiology and Immunology
Montana State University
Bozeman, MT 89717
E-mail:eschmidt@montana.edu



Research Interests

Gene Regulation, Physiology, Metabolism, Development

Research Summary

The Schmidt Lab studies intricate gene regulatory mechanisms that function in the development and maintenance of complex organisms like ourselves. Most of our work is based on analyses of mouse lines we produce that bear targeted mutations (e.g. "knockouts"); however, our approaches to understanding the roles of the mutated genes are broad, including phylogenetics, genetics, biochemistry, molecular biology, histology, biophysics, genomics, proteomics, and other techniques. The biological processes we are studying include early embryonic patterning and development, placental development, metabolism, redox homeostatis, stress responses, stem cell biology, immune cell functions, and the maternal/fetal immune interaction.

Research Publications

Sonsteng KM, Prigge JR, Talago EA, June RK, Schmidt EE. Hudrodynamic delivery of Cre protein to lineage-mark or time-stamnp mouse hepatocytes in situ. PLoS One. 2014 March 13:9(3):e91219. 

Gorrini C, Gang BP, BAssi C, Wakeham A, Baniasadi SP, Hao Z, Li WY, Cescon DW, Li YT, Molyneux S, Penrod N, Lupien M, Schmidt EE, Stambolic V, Gauthier ML, Mak TW. Estrogen controls the survival of BRCA1-deficient cells via a PI3K-NRF2-regulated pathway. Proc. Natl. Acad Sci USA. 2014 Mar 25:111(12):4472-7. 

Prigge JR, Wiley JA, Talago EA, Young EM, Johns LL, Kundert JA, Sonsteng KM, Halfor WP, Capecchi MR, and Schmidt EE: Nuclear double-flourescent reporter for in vivo and ex vivo analyses of biological transitions in mouse nuclei. 2013, Mamm Genome, 24:389-99. 

Iverson SV, Eriksson S, Xu J, Prigge JR, Talago EA, Meade TA, Meade ES, Capecchi MR, Arnér ESJ, and Schmidt, EE: A Txnrd1-dependent metabolic switch alters hepatic lipogenesis, glycogen storage, and acetaminophen susceptibility. 2013, Free Rad Biol 63:369-80.

Huebner AJ, Dai D, Morasso M, Schmidt EE, Schäfer M, Werner S, Roop DR. Amniotic fluid activiates the Nrf2/Keap 1 pathway to repair an epidermal barrier defect in utero. Dev. Cell, In press (accepted 15 October 2012). 

Locy ML, Rogers LK, Prigge JR, Schmidt EE, Arnér E, Tipple TE. Tioredoxin reductase inhibition elicits Nrf2-mediated responses in clara cells: implications for oxidant-induced lung injury. Antioxid. Redox Signal. 2012. 17(10) 1407-16. 

Prigge JR, Eriksson S, Iverson SV, Meade T, Capecchi MR, Arnér ESJ, and Schmidt EE: Hapatocyte replication in growing liver requires either flutathione or a single allele of txnrdl. Free Rad. Biol. Med., 2012, 52:803-812. 

Iverson SV, Comstock CM, Kundert JA, and Schmidt EE: Contributions of new hepatocyte lineages to liver growth, maintenance, and regeneration in mice. Hepatology 2011, 64(2)655-663. 

Liu M, B. Rakowski, et al. (2010). “ICP0 antagonizes ICP4-dependent silencing of the herpes simplex virus ICP0 gene.” PloS One 5(1):e8837.

Liu M, EE Schmidt, et al. (2010). “ICP0 dismantles microtubule networks in herpes simples virus-infected cells.” PloS One 5(6):e10975. 

Rollins MF, DM van der Heide, et al. (2010). “Hepatocytes lacking thioredoxin reductase 1 have normal replicate potential during development and regeneration.” Journal of cell science 123(Pt 14):2402-2412. 

Weisend CW, JA Kundert, ES Suvorova, JR Prigge, and EE Schmidt, Cre activity in fetal albCre mouse hepatocytes: Utility for developmental studies. Genesis. 2009. In press. 

Prigge JR, SV Iverson, AM Siders, and EE Schmidt, Interactome for auxiliary splicing factor U2AF(65) suggests diverse roles. BBA Gene Reg Mech 2009. 1789:p. 487-92.

Suvorova ES, O Lucas, CM Weisend, MF Rollins, GF Merrill, MR Capecchi, and EE Schmidt,Cytoprotective Nrf2 pathway is induced in chronically txnrd 1-deficient hapatocytes. PloS One, 2009. 4(7):p. e6158. 

Kundert JA, AL Sealey, Y Li, MR Capecchi and EE Schmidt, Syngeneic immune-dependent abortions in mice suggest paternal alloantigen-independent mechanism. Am J Reprod Immunol, 2008. 60(4):p.290-7. 

Bondareva AA, MR Capecchi, SV Iverson, Y Li, NI Lopez, O Luca, GF Merrill, JR Prigge, AM Siders, M Wakamiya, SL Wallin and EE Schmidt, Effects of thioredoxin reductase-1 deletion on embryogenesis and transcriptome. Free Radic Biol Med, 2007. 43(6): p. 911-23. 

Prigge JR and EE Schmidt, HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP(CORE). BMC Mol Biol, 2007. 8:p. 76. 

Sansinena MJ, SA Taylor, PJ Taylor, EE Schmidt, RS Denniston, and RA Godke (2007). In vitro production of llama (Lama Glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions. An. Reprod. Sci. 99, 342-353. 

Schmidt EE and CJ Davies, The origins of polypeptide domains. Bioessays, 2007. 29(3):p. 262-70.

Prigge JR and EE Schmidt, Interaction of protein inhibitor of actvated STAT (PIAS) proteins with the TATA-binding protein, TBP. J Biol Chem, 2006. 281(18):p. 12260-9. 

Tucker TA, JA Kundert, AA Bondareva and EE Schmidt, Reproductive and neurological Quaking (viable) phenotypes in a severe combined immune deficient mouse background. Immunogenetics, 2005. 57(3-4): p. 226-31. 

Hobbs NK, AA Bondareva, S Barnett, MR Capecchi, and EE Schmidt (2002). Removing the vertebrate specific TBP N terminus disrupts placental beta-2m-dependent interactions with the maternal immune system. Cell 110, 43-54. 

Schmidt EE, Bondareva AA, Radke JR and Capecchi MR (2003) Fundamental cellular processes do not require vertebrae-specific sequences within the TATA-bonding protein, TBP. J. Biol. Chem. 278, 6168-6174. 

Bondareva AA and EE Schmidt (2003). Early vertebrate evolution of the TATA-binding protein. Mol. Biol. Evol. 20, 1932-1939.

Washington State University