|Department:||School of Biological Sciences, WSU|
|Credentials:||1977~Ph.D., University of Washington|
School of Biological Sciences
Fish Genetics and Reproduction
Research in our laboratory involves genetic studies in trout and salmon, with special emphasis on rainbow trout. We apply the chromosome set manipulation techniques of gynogenesis and androgenesis to produce clonal lines. Clonal lines of rainbow trout are being used for genetic mapping and with the goal of identifying major genes affecting adaptive and economically important traits. By analyzing inheritance of DNA markers and traits of interest in controlled matings, we can assess where major genes are located on the trout genetic map. Another of our interests is the genetic basis of sex determination in trout and salmon. We are collaborating with researchers who are using the clonal lines for reproductive biology, immunology and physiological research. Studies using DNA markers and chromosome polymorphisms are also being used to assess relationships among salmonid populations to address conservation concerns.
Selected Publications 2008-2013
Pulcini, D., P.A. Wheeler, S. Cataudella, T. Russo and G.H. Thorgaard, 2013. Domestication shapes morphology in rainbow trout Oncorhynchus mykiss. J. Fish Biol. : in press.
Brunelli, J.P., J.M. Mallatt, R.F. Leary, M. Alfaqih, R.B. Phillips and G.H. Thorgaard, 2013. Y chromosome phylogeny for cutthroat trout (Oncorhynchus clarkii) subspecies is generally concordant with those of other markers. Mol. Phylo. Evol. 66: 592-602.
Robison, B.D. and G. H. Thorgaard, 2012. Prospects and pitfalls of clonal fishes in the post-genomic era. In: G. L. Fletcher and M. Rise, editors. Aquaculture Biotechnology. Wiley-Blackwell , pp. 55-67.
Miller, M.R., J.P. Brunelli, P.A. Wheeler, S.Liu, C.E. Rexroad III,Y. Palti, C.Q. Doe and G.H. Thorgaard, 2012. A conserved haplotype controls parallel adaptation in geographically distant salmonid populations. Mol. Ecol. 21: 237-249.
Wargo, A.R., A.M. Kell, R.J. Scott, G.H. Thorgaard and G. Kurath, 2012. Analysis of host genetic diversity and viral entry as sources of between-host variation in viral load. Virus Research 165: 71-80.
Xu, P., L. McIntyre, J. Scardina, P. Wheeler, G. Thorgaard and K. Nichols, 2011. Transcriptome profiling of embryonic development rate in rainbow trout advanced backcross introgression lines. Marine Biotechnology 13:215-31
Hale, M.C. P. Xu,, J. Scardina, P.A. Wheeler, G.H. Thorgaard and K.M. Nichols, 2011. Differential gene expression in male and female rainbow trout embryos prior to the onset of gross morphological differentiation of the gonads. BMC Genomics12:404.
Brunelli, J. P., C. A. Steele, et al. (2010). "Deep divergence and apparent sex-biased dispersal revealed by a Y-linked marker in rainbow trout." Molecular phylogenetics and evolution 56(3): 983-990.
Salem, M., C. E. Rexroad, 3rd, et al. (2010). "Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches." BMC genomics 11: 564.
Alfaqih, M.A., J.P. Brunelli, R.E. Drew, and G.H. Thorgaard, Mapping of five candidate sex-determining loci in rainbow trout (Oncorhynchus mykiss). BMC Genet, 2009. : p. 2.
Purcell, M.K., K.J. Laing, J.C. Woodson, G.H. Thorgaard, and J.D. Hansen, Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes. Fish Shellfish Immunol, 2009. 26(2): p. 293-304.
Alfaqih, M.A., R.B. Phillips, P.A. Wheeler, and G.H. Thorgaard, The cutthroat trout Y chromosome is conserved with that of rainbow trout. Cytogenet Genome Res, 2008. 121(3-4): p. 255-9.
Brunelli, J.P., K.J. Wertzler, K. Sundin, and G.H. Thorgaard, Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon. Genome, 2008. 51(9): p. 739-48.
Heredia-Middleton, P., J. Brunelli, R.E. Drew, and G.H. Thorgaard, Heat shock protein (HSP70) RNA expression differs among rainbow trout (Oncorhynchus mykiss) clonal lines. Comp Biochem Physiol B Biochem Mol Biol, 2008. 149(4): p. 552-6.
Landis, E.D., M.K. Purcell, G.H. Thorgaard, P.A. Wheeler, and J.D. Hansen, Transcriptional profiling of MHC class I genes in rainbow trout infected with infectious hematopoietic necrosis virus. Mol Immunol, 2008. 45(6): p. 1646-57.
Nichols, K.M., A.F. Edo, P.A. Wheeler, and G.H. Thorgaard, The genetic basis of smoltification-related traits in Oncorhynchus mykiss. Genetics, 2008. 179(3): p. 1559-75.
Wibowo, T.A., C.T. Gaskins, R.C. Newberry, G.H. Thorgaard, J.J. Michal, and Z. Jiang, Genome assembly anchored QTL map of bovine chromosome 14. Int J Biol Sci, 2008. 4(6): p. 406-14.