Participating Faculty
John Alderete
Name:John Alderete
Department:School of Molecular Biosciences
Credentials:1978-Ph.D. University of Kansas - Microbiology
Office:Biotechnology Life Sciences 437
Phone:509-335-8125; Lab-509-335-8042
Fax:509-335-4154
Mailing Address:

PO Box 647520
Pullman, WA 99164-7520

E-mail:alderete@vetmed.wsu.edu



Research Interests

The focus of our area of research is the characterization at the molecular level of the mechanisms of pathogenesis for the number one sexually transmitted infection caused by the parasite Trichomonas vaginalis. We are studying the biology of the host-parasite interrelationship and identifying editopes of immunogenic virulence factors that are candidates for Point-of-Care serodiagnostics for both women and men.

Research Summary

The following represent recent noteworthy accomplishments: 1) Membrane proteins that mediate cytoadherence to vaginal epithelial cells have been identified. 2) Significant membrane remodeling results from distinct signaling pathways involving iron and contact with host cells. 3) Numerous metabolic enzymes were discovered to be secreted and become anchorless, surface-associated proteins with alternative functions on the parasite surface. This functional diversity is evidence by the enzymes have specific receptor binding for host proteins that then become pathogenicity factors. For example, GAPDH is the receptor for fibronectin, and α-enolase is the receptor for plasminogen. 4) A Point-of-Care diagnostic (sold by Sensui, Inc.) was invented, and this diagnostic was the direct of basic research studying the property of adherence.

Recent Patent:

Patent No. 61/779, 166, “Methods and compositions to diagnose Trichomonas vaginalis infection.” Inventor J.F. Alderete, Provisional Patents submitted 13 March 2013

Representative Publications:
Ibanez-Escribano, A., et al. (2014). "Determination of internal transcribed spacer regions (ITS) in Trichomonas vaginalis isolates and differentiation among Trichomonas species." Parasitol Int 63(2): 427-431.

Neace, C., and J.F. Alderete. 2013. Epitopes of the highly immunogenic Trichomonas vaginalis α-actinin are serodiagnostic targets for both women and men. J. Clin. Microbiol. 51:2483-2490. PMCID: 23616456.

Neace, C., and J.F. Alderete. 2013. Identification, Characterization, and Synthesis of Peptide Epitopes and A Recombinant Six-Epitope Protein for Trichomonas vaginalis diagnosis. Immuno Targets and Therapy, 2:91-103.

Ibanez-Escribano, A., J.J. Noal-Ruiz, V.J. Aran, J. Antonio-Escario, A. Gomez-Barrio, and J.F. Alderete. 2013. Determination of Internal Transcribed Spacer regions (ITS) in Trichomonas vaginalis isolates and differentiation among Trichomonas species. Int’l J. Parasitology. In press, April issue.

Chen, Y-C., Y.-L. Huang, E.A. Platz, J.F. Alderete, L. Zheng, J.R. Rider, P. Kraft, E. Giovannucci, and S. Sutcliffe. 2013. Prospective study of the influence of Toll-like receptor 4 variation on the association between Trichomonas vaginalis serostatus and prostate cancer. Carcinogeneisis. Cancer Causes Control. 24:175-180. PMCID: 23179660.

Sutcliffe, S., C. Neance, N. Magnuson, R. Reeves, and J.F. Alderete. 2012. Trichomonas vaginalis, a common non-viral STI and prostate cancer-a proposed molecular mechanism. PLoS Pathogens, Aug;8(8):e1002801, Epub 2012 August 9. PMCID: 22912571.

Huang, K.-Y., P.-J. Huang, F.-M. Ku, R. Lin, P. Tang, and J.F. Alderete. 2012. Comparative transcriptomics and proteomic analyses of Trichomonas vaginalis following adherence to fibronectin. Infect. Immun. 80:3900-3911. PMCID: 22927047.

Hernandez-Romano, P., R. Arroyo, J.F. Alderete, R. Hernandez, and I. Lopez-Villasenor. 2010. Identification and characterization of a surface-associated subtilisin-like serine protease in Trochomonas vaginalis. Parasitology. 137:1621-1635. PMCID: 20602853.

Nogueira, S.V., M.L. Rodrigues, V. Mundodi, E.A. Abi-Chacra, M.S. Winters, J.F. Alderete, and C.M. de Almeida Soares, 2010. Paracoccidioides brasiliensis enolase is a surface protein that binds plasminogen and mediates interaction of yeast forms with host cells. Infect. Immun. 78:4040-4050. PMCID: 20605975.

Kucknoor, A.S., V. Mundodi, and J.F. Alderete. 2009. Genetic identity and differential gene expression between Trichomonas vaginalis and T. tenax. BMC Microbiol. 9:58 (18 March 2009) http://www.biomedcentral.com/1471-2180/9/58. PMCID: 19296850.

Torres-Macharro, A.L., R. Hernandez, J.F. Alderete, and I. Lopez-Villasenor. 2009. Comparative analyses among the Trichomonas vaginalis, Trichomonas tenax, and Tritrichomonas foetus 5S ribosomal RNA genes. Current Genetics. 55:199-210. PMCID: 19290527.

Lama, A., A.S. Kucknoor, V. Mundodi, and J.F. Alderete. 2009. Glyceraldehyde-3-phosphate dehydrogenase is a surface-associated fobronectin-binding protein of Trichomonas vaginalis. Infect. Immun. 77:2703-2711. PMCID: 19380472.

Sutcliffe, S., J.F. Alderete, C. Till, P.J. Goodman, A.W. Hsing, J.M. Zenilman, A.M. DeMarzo, and E.A. Platz. 2009. Trichomonisis and subsequent risk of prostate cancer in the prostate cancer prevention trial. Int’l J. Cancer. 124:2082-2087. PMCID: 19117055.

Gerhold, R.W., A.B. Allison, H. Sellers, E. Linnemann, T.-H. Chang, and J.F. Alderete. 2009. Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus. Parasitol. Res. 105:775-779. PMCID: 19452169.

Sutcliffe, S., I. Kawachi, J.F. Alderete, C.A. Gaydos, L.P. Jacobson, F.J. Jenkins, R.P. Viscidi, J.M. Zenilman, and E.A. Platz. 2009. Correlates of sexually transmitted infection histories in a cohort of American male health professionals. Cancer Causes & Control. 20:1623-1634. PMCID: 19655261.

Stark, J. R., et al. (2009). "Prospective study of Trichomonas vaginalis infection and prostate cancer incidence and mortality: Physicians' Health Study." J Natl Cancer Inst 101(20): 1406-1411.

Stark, J.R., G. Judson, J.F. Alderete, V. Mundodi, A. Kucknoor, E.L. Giovannucci, E.A. Platz, S. Sutcliffe, K. Fall, T. Kurth, J. Ma, M. Stampfer, and L. Mucci. 2009. Trichomonas vaginalis infection and prostate cancer incidence and mortality: a prospective study in the physician’s health study. J. Nat. Cancer Inst. 101:1-6. PMCID: 19741211.

 Center for Reproductive Biology is cited as author affiliation.

Washington State University