|Department:||School of Molecular Biosciences, WSU|
|Credentials:||1982 - Ph.D., Princeton University|
|Office:||Biotechnology Life Sciences 345|
|Mailing Address:||School of Molecular Biosciences|
Washington State University
PO Box 647520
Pullman, WA 99164-7520
Testis Biology/Reproductive Toxicology
The primary goal of my research is to understand the molecular and cellular mechanisms controlling embryonic and postnatal testis development and sperm production. The first research focus is to determine the role of vitamin A (retinoids) and retinoic acid receptors in stem cell function and meiosis. We are characterizing retinoic acid receptor alpha mutant mice with specific deletion of the receptor in germ cells. The second research focus is to determine the transgenerational mechanism by which phthalate plasticizers, environmental toxins, interfere with stem cell function and meiosis in the testis. To do these, we use state-of-the-art molecular and cellular techniques and in vivo animal model systems such as animals going through embryonic and neonatal development, environmental toxin-treated or vitamin A-deprived rats and mice, and transgenic receptor knockout mice.
Doyle TJ, Bowman JL, Windell VL, McLean DJ, KH Kim (2013). Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice. Biol. Reprod. 88:112-126. Faculty of 1000 Prime Recommended.
Doyle TJ, Kaur G, Putrevu SM, Dyson M, McCunniff WT, Pasham MR, Kim KH, JM Dufour (2012). Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege. Biol. Reprod. 86:1-14.
Santos NC and KH Kim (2010). Activity of retinoic acid receoptor-alpha is directly regulated at its protein kinase A sites in response to follicle-stimulating hormone signaling. Endocrinology 151:2361-2372.
Zhu L, TJ Doyle and KH Kim (2010). Retinoic acid modulates the subcellular localization of small ubiquitin-related modifier-2/3 (SUMO-2/3) in the testis. J. Androl. 31:406-418.
Zhu L, NC Santos and KH Kim (2010). Disulfide isomerase glucose-regulated protein 58 is required for the nuclear localization and degradation of retinoic acid receptor alpha. Reproduction 139:717-731.
Zhu L, NC Santos and KH Kim (2009). Small ubiquitin-like modifier-2 modification of retinoic acid receptor-alpha regulates its subcellular localization and transcriptional activity. Endocrinology 150:5586-5595.
Doyle TJ, AJ Oudes and KH Kim (2009). Temporal profiling of rat transcriptomes in retinol-replenished vitamin A-deficient testis. Syst. Biol. Reprod. Med. 55:145-163.
Doyle TJ, KW Braun, DJ McLean, RW Wright, MD Griswold and KH Kim (2007). Potential funcitons of retinoic acid receptor A in Sertoli cells and germ cells during spermatogenesis. Ann. N.Y. Acad. Sci. 1120:114-130.
Bhattacharya N, JM Dufour, MN Vo, J Okita, R Okita and KH Kim (2005). Differential effects of phthalates on the testis and the liver. Biol. Reprod. 72:745-754.
Li H and KH Kim (2004). Retinoic acid inhibits rat XY gonad development by locking mesonepheric cell migration and decreasing the number of gonocytes. Biol. Reprod. 70:687-693.
Li H and KH Kim (2003). Effects of mono- (2-ethylhexyl) phthalate on fetal and neonatal rat testes in organ cultures. Biol. Reprod. 69:1964-1972.
Li H and KH Kim (2003). Effects of ethanol on embryonic and neonatal rat testes in organ cultures. J. Andro. 24:653-660.