Male Reproduction/Sperm Maturation
Successful fertilization of the egg requires sperm capable of motility and possessing the ability to undergo an acrosome-reaction upon contact with the egg; defects in these sperm functions contribute significantly to male infertility. Sperm gain the ability to swim and to fertilize an oocyte during their transit through the epididymis. Epididymal sperm maturation is characterized by changes in both protein and lipid composition of the sperm. The research in my laboratory investigates the molecular function of sperm maturation, with specific focus on how lipid synthesis and maintenance occur. The laboratory employs various molecular biology and biochemical techniques to investigate the maturation process, with hopes of understanding male infertility and possibly finding a contraceptive target.
Chauvin, T. R., et al. (2012). "Cold-shock-domain protein A (CSDA) contributes posttranscriptionally to gonadotropin-releasing hormone-regulated expression of Egr1 and indirectly to Lhb." Biol Reprod 86(2): 53.
Chauvin TR, Xie F, Liu T, Nicora CD, Yang F, Camp II DG, Smith RD, and Roberts KA. Systematic Analysis of a Deep Mouse Epididymal Sperm Proteome. Biology of Reproduction 2012; 87:141, 1-8.
Chauvin TR, Herndon MK and Nilson JH. Cold-Shock-Domain Protein A (CSDA) Contributes Post-Transcriptionally to Gonadotropin-Releasing Hormone-Regulated Expression of Egr1 and Indirectly to Lhb. Biology of Reproduction 2011; 86:53, 1-10.
Chauvin TR and Griswold MD. Identification of Regulated Genes in the Murine Epididymis Using Oligonucleotide Microarrays. Biology of Reproduction 2004; 71:560-569.
Chauvin TR and Griswold MD. Characterization of the Expression and Regulation of Genes Necessary for myo-Inositol Biosynthesis and Transport in the Seminiferous Epithelium. Biology of Reproduction, 2004; 70:744-751.
Chauvin, T. R. and M. D. Griswold (2004). "Androgen-regulated genes in the murine epididymis." Biol Reprod 71(2): 560-569.