Plant Reproduction Biology; Flower and Seed Development; Cytoplasmic Male Sterility; Callose Biosynthesis; Symbiotic Nitrogen Fixation
Formation of viable pollens is essential for sexual reproduction of flowering plants, and vital to grain production in agriculture. During pollen formation, a callose wall is built on the pollen mother cells and microspore tetrads. The callose wall is degraded by beta-1, 3-glucanase (callase) secreted by the tapetum cells, leading to the release of microspores in the locular space of developing anthers. Screens of Arabidopsis mutants defective in the callose wall have identified a group of genes required for the formation of viable pollens. Research in my laboratory focuses on the characterization of callose synthases that are responsible for the synthesis of callose during pollen development. We have also been characterizing genes that are responsible for grain development in wheat, barley and rice. The experimental approach taken in my laboratory includes biochemistry, molecular biology, cell and developmental biology, genetics, genomics and proteomics.
Mjomba FM, Zheng Y, Liu H, Tang W, Hong Z, Wang F, Wu W (2016) Homeobox is pivotal for OsWUS controlling tiller development and female fertility in rice. Genes, Genomics and Genetics (G3, Bethesda) 6: 2013-2021.
Wang L, Wang L, Tan Q, Fan Q, Zhu H, Hong Z, Zhang Z, Duanmu D (2016) Efficient inactivation of symbiotic nitrogen fixation related genes in Lotus japonicas using CRISPR-Cas9. Front Plant Sci 7: 1333.
Wang C, Yu H, Luo L, Duan L, Cai L, He X, Wen J, Mysore KS, Li G, Xiao A, Duanmu D, Cao Y, Hong Z, Zhang Z (2016) NODULES WITH ACTIVATED DEFENSE 1 is required for maintenance of rhizobial endosymbiosis in Medicago truncatula. New Phytol 212: 176-191.
Wang C, Yu H, Zhang Z, Yu L, Hong Z, Luo L (2015a) Phytosulfokine is involved in positive regulation of Lotus japonicus nodulation. Mol Plant-Microbe Interact 28: 847-855
Wang C, Xu X, Hong Z, Feng Y, Zhang Z (2015b) Involvement of ROP6 and clathrin in nodulation factor signaling. Plant Signaling & Behavior 10(7):e1033127
Wang C, Zhu M, Duan L, Yu H, Chang X, Li L, Kang H, Feng Y, Zhu H, Hong Z, Zhang Z (2015c) Lotus japonicus clathrin heavy chain 1 is associated with ROP6 GTPase and involved in nodule formation. Plant Physiol 167: 1497-1510
Kang H, Xiao A, Huang X, Gao X, Yu H, He X, Zhu H, Hong Z, Zhang Z (2015) A Lotus japonicus co-chaperone protein interacts with the ubiquitin-like domain protein CIP73 and plays a negative regulatory role in nodulation. Mol Plant-Microbe Interact 28: 534-545
Hu G, Burton C, Hong Z (2014) Molecular and chemical characterization of a new waxy allele in barley (Hordeum vulgare L.). Cereal Chem 91: 438-444
Chen, F, Jiang L, Zheng J, Huang R, Wang H, Hong Z, Huang Y (2014) Identification of differentially expressed proteins and phosphorylated proteins in rice seedlings in response to strigolactone treatment. PLoS One 9(4): e93947.
Hu G, Burton C, Hong Z, Jackson E (2014) A mutation of the cellulose-synthase-like (CsIF6) gene in barley (Hordeum vulgare L.) partially affects the beta-glucan content in grains. J Cereal Sci 59: 189-195.
Kang H, Chu X, Wang C, Xiao A, Zhu H, Yuan S, Yang Z, Ke D, Xiao S, Hong Z, Zhang Z (2014) A MYB coiled-coil transcription factor interacts with NSP2 and is involved in nodulation in Lotus japonicas. New Phytol 201:837-849.
Huang R, Jiang L, Zheng J, Wang T, Wang H, Huang Y, Hong Z (2013) Genetic bases underlying rice grain shap: So many genes, so little known. Trends Plant Sci 18:218-226
Wang C, Zhu H, Jin L, Chen T, Wang L, Kang H, Hong Z, Zhang Z (2013) Splice variants of the SIP1 transcripts play a role in nodule organogenesis in Lotus japonicas. Plant Mol Biol 82:97-111.
Chen F, Jiang L, Zheng J, Huang R, Wang H, Hong Z, Huang Y (2013) Identification of a cosegregative protein associated with the tillering trait in rice (Oryza sativa L. indica). Plant Omics J 6:36-45.
Wang X, Gingrich DK, Deng Y, Hong Z (2012) NSN1, a nucleostemin-like GTPase required for apical and floral meristem development in Arabidopsis. Mol Biol Cell 23:1446-1456.
Wang X, Xie B, Zhu M, Zhang Z, Hong Z (2012) Nucleostemin-like 1 is required for embryogenesis and leaf development in Arabidopsis. Plant Mol Biol 78: 31-44
Chen T, Zhu H, Ke D, Cai K, Wang T, Gou H, Hong Z, Zhang Z. (2012) A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicas. Plant Cell 24:823-838.
Ke D, Fang Q, Chen C, Zhu H, Chen T, Chang X, Yua Sa, Ma L, Hong Z, Zhang Z (2012) Small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicas. Plant Physiol 159:131-143.
Yuan S, Zhu H, Gou H, Fu W, Liu L, Chen T, Ke D, Kang H, Xie Q, Hong Z, Zhang Z (2012) A ubiquitin ligase of symbiosis receptor kinase involved in nodule organogenesis. Plant Physiol 160:106-117.
Xie B, Hong Z (2011) Unplugging the callose plug from sieve pores. Plant Signaling & Behavior, 6:491-493.
Xie B, Wang X, Zhu M, Zhang Z, Hong Z (2011a) CalS7 encodes a callose synthase responsible for callose deposition in the phloem. Plant J 65:1-14.
Xie B, Deng Y, kanaoka M, Okada K, Hong Z (2011b) Expression of Arabidopsis callose synthase 5 (CalS5) in tobacco BY-2 cells: subcellular localization and effect on cell wall permeability. Plant Sci 183:1-8.
Kang H, Zhu H, Chu X, Yang Z, Yuan S, Yu D, Wang C, Hong Z, Zhang Z (2011) A novel interaction between CCaMK and a protein containing the Scythe_N ubiquitin-like domain in Lotus japonicas. Plant Physiol 155:1312-1324. PMID: 21209278
Guseman JM, Lee JS, Bogenschutz NL, Peterson KM, Virata RE, Xie B, Kanaoka MM, Hong Z, Torii KU (2010) Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis CHORUS (GLUCAN SYNTHASE-LIKE 8). Development 137:1731-1741.
Xie B, Wang X, Hong Z (2010) Precocious pollen germination in Arabidopsis lines with altered callose deposition during microsporogenesis. Planta 231:809-823