Core Laboratories

Core Laboratories

The Center has 5 core laboratories, which help integrate research and provide services required by all the investigators. These cores reduce redundancy between labs and provide services not all the individual labs routinely perform.

Click on the Name of any Core Laboratory for More Information about that Core.



Administrative Core

Administrative Core:

Directed by Dr. Jon Oatley, this core integrates the projects by organizing seminars and the annual retreat. The Administrative Core oversees all fiscal management of the program and cores, and facilitates university and agency interactions. This core provides general administrative services, in particular the interactions both in regards to budgets and services of the other cores.

Contact:
Coordinator/Administrative Manager: Carolyn Emerson-Farr; 509-335-2473; emersonc@wsu.edu

Director: Jon Oatley; 509-335-0499; joatley@wsu.edu



Transgenic Core

Animal Production Core

The Animal Production Core (APC) offers a range of services for generating and preserving gene-edited and genetically modified animal models for investigators at WSU and beyond. Embryo microinjection and micromanipulation services are available for generation of transgenic, knock-out, knock-in, or otherwise genetically engineered animals, using zygote or blastocyst injection with DNA, RNA, CRISPR-Cas9 reagents, or ES cells. The Animal Production Core works in close collaboration with the Gene Editing Reagent Core (GERC) to provide full-service genome editing services, from design of targeting strategies to delivery of founder animals to investigators. In addition, the APC can provide a range of traditional transgenic core services, with experience and expertise in embryo transfer surgeries, embryo and sperm cryopreservation and cryorecovery, rederivation of specific-pathogen-free lines, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and other reproductive biology techniques. Well-established protocols are in place for working with mouse and rat embryos and gametes, and we are happy to work with other laboratory animal models and large animal species as well. Projects from investigators outside WSU are also welcome, and we are happy to discuss possible research collaborations.



FACS Core

FACS Core

Directed by Dr. Jon Oatley, the Fluorescent Activated Cell Sorting (FACS) core provides a fee based service for investigators to isolate subsets of cells from a heterogonous population based on up to 8 different parameters.



Tyrosinase CRISPR mutants test

Gene Editing Reagent Core:

Directed by Dr. Lisette Maddison, the Gene Editing Reagent Core (GERC) provides a fee based service to provide CRISPR/Cas9 reagents for genome manipulation in many species. The mission of the Core is to help investigators integrate cutting edge genome manipulation into their research. Reagents will be developed in close consultation with the investigators for personalized results. Available services include individual guide RNA and Cas9 supplied separately or cloned into plasmid vectors and repair templates can be generated for precise genomic changes from small to large.



Molecular Biology Core and Genomics Core

Molecular Biology Core and Genomics Core:

Co-Directed by Michael Griswold and Derek Pouchnik, these Cores provide DNA sequencing, protein sequencing, oligonucleotide synthesis, peptide synthesis, and some information services to members of the Center for Reproductive Biology. A state of the art core laboratory providing all genomics micro-array technology equipment including access to an Affymetrix Micro-Array apparatus and data analysis system, as well as a custom array apparatus for glass slide array of investigator initiated arrays. The Core has the bio-informatics support for the procedures performed and assists the investigators in data analysis. Technical support is provided to perform the analysis on the Affymetrix chips and in the production of the custom arrays.



Shared Resources

Shared Resources

The Center has continued support of services offered by closed Cores. Specialized equipment and training are available for faculty and trainees to use. Equipment and training are offered free of charge. Media/supplies used will be charged to the PI.

Washington State University