Molecular Biology and Genomics Core

Co-Directors: Dr. Michael Griswold and Derek Pouchnik


Summary:

Molecular Biology and Genomics CoreThe WSU Molecular Biology and Genomics Core began in the early 1990s and is housed withing the Laboratory for Biotechnology and Bioanalysis. This core was established to allow individual investigators local access to state of the art molecular biology and Next Generation DNA sequencing tools. Over the past 20 years, with the assistance of the Center for Reproductive Biology, M.J. Murdock Charitable Trust, NIH, and others, the core lab has evolved into a state-of-the-art service facility.

The core lab is directed by Dr. Michael Griswold in the School of Molecular Biosciences and currently has three full time personnel.  The facilities are housed in two laboratories in the Biotechnology and Life Sciences Building (rooms 127 and 227). We have a multitude of services and instrumentation available in the fields of molecular biology, genomics, and proteomics.


Services and Instruments:

We currently offer services for Sanger DNA sequencing and Genotyping (fragment analysis) on our ABI 3730 and we have two real time PCR instruments (ABI 7500SDS) are available for qPCR and genotyping applications.

Additionally, the Core runs and maintains two Next Generation DNA sequencing platforms, the Ion Proton, and the Pacific Biosciences Sequel. Library and sample preparation services for these platforms as well as data acquisition are offered and some bioinformatics support is available for specific applications.

Resources:

  • ABI 3730 DNA Analyzer
  • Agilent 5200 Fragment Analyzer
  • Applied Biosystems 7500fast Real Time PCR (x2)
  • Ion Proton, Life Technologies
  • PacBio Sequel, Pacific Biosciences
  • Takara High Throughput qPCR/Genotyping

Cost of Services:

Please see the LBB pricing website or contact LBB for more information.

CONTACT:

Laboratory of Biotechnology & Bioanalysis
227 Biotechnology/Life Sciences Building
Pullman, WA 99164-7520
509-335-1174
lbb@wsu.edu

Lab hours:
8:00am - 5:00pm; Monday - Friday

For more information on all services please contact:
https://lbb.wsu.edu/

Derek Pouchnik
509-335-1174
pooch@wsu.edu

Mark Wildung
wildung@wsu.edu

Lab Staff
Derek Pouchnik
Mark Wildung
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