Gene Editing Reagent Core,
Director Center for Reproductive Biology
Room: Biotechnology/Life Sciences 141
My broad interest is in generating new research models using cutting edge techniques. This includes CRISPR based applications or other approaches to manipulate the genome.
Biological systems have great complexity and whole organism research models help to capture a better understanding of critical factors and processes. As director of the Gene Editing Reagent Core (GERC) I’m interested in using the most relevant techniques to help investigators incorporate tailored genetic models into their research. The GERC is positioned to help investigators incorporate the state of the art gene editing and other genetic manipulation approaches into their scientific endeavors. Gene editing has been greatly facilitated by CRISPR/Cas9 and allows manipulation of the genomic DNA in virtually any species. CRISPR applications include generating mutations leading to a functional knockout, precision editing where a single amino acid can be altered, and knock-in approaches to include new elements into the gene of interest. My scientific career has revolved around building and using animal models; from transgenic models including those for tissue and temporal specific gene manipulation, retroviral based mutagenesis, to new models developed using TALEN and CRISPR. I’ve used these models to investigate research questions from cancer progression to pancreatic endocrine cell function. I’m interested in working with scientists across the WSU system to help elevate their research programs by incorporating similar approaches.
- Yin L1, Maddison LA1, Chen W1. (2016) Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system. Methods Cell Biol. 135:3-17. doi: 10.1016/bs.mcb.2016.04.018. Epub 2016 May 30. PMID: 27443918 PMCID:
- Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W. (2015) Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics. PMID: 25855067 PMCID: PMC4492370